Role of alcohol layer in dna isolation?
DNA molecules are surrounded by water molecules when in solution. This surrounding water is called the shell of hydration. Alcohol is a dehydrating agent. When alcohol is added (along with certain salts) during DNA isolation, it results in the disruption of the hydration shell. At this stage, the DNA comes out of solution and is said to be in a precipitated state. Precipitated DNA can then be separated from the remaining solubilized components through s process called centrifugation.
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